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Beyotime
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Image Search Results
Journal: iScience
Article Title: The role of mitofusin 2 in regulating endothelial cell senescence: Implications for vascular aging
doi: 10.1016/j.isci.2024.110809
Figure Lengend Snippet:
Article Snippet: β-Gal staining kit ,
Techniques: Virus, Recombinant, Bicinchoninic Acid Protein Assay, Staining, Gene Expression, Western Blot, Plasmid Preparation, Software
Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Disturbed glycolipid metabolism activates CXCL13-CXCR5 axis in senescent TSCs to promote heterotopic ossification
doi: 10.1007/s00018-024-05302-3
Figure Lengend Snippet: Disturbed glycolipid metabolism promoted TSC senescence and enhanced osteogenesis. a . The p16 staining of the Achilles tendons of Control mice and DM mice and their quantification. Scale bar = 100 μm. b . The β-Gal staining of nTSCs and dTSCs and their quantification. Scale bar = 50 μm. c . The β-Gal staining of TSCs cultured in low glucose medium and HFHG medium and their quantification. Scale bar = 50 μm. d . The expression of mRNA relevant to SASP of TSCs cultured in low glucose medium and HFHG medium. e . Protein levels of cyclin D1, cyclin B1, and p21 of TSCs cultured in low glucose medium and HFHG medium. f . The β-Gal staining of TSCs cultured in 0, 150, 225, or 300 µM H 2 O 2 and their quantification. Scale bar = 50 μm. g . ALP staining and alizarin red staining (left) and the protein levels of TNMD, RUNX2, and OPN (right) of TSCs cultured in 0, 150, 225, or 300 µM H 2 O 2 . Scale bar = 1 mm. h . Protein levels of OCT4, NANOG, and p21 of TSCs after 3 times passage (P3) and 10 times passage (P10). i . Protein levels of RUNX2 and OPN (left) and ALP staining and alizarin red staining (right) of P3 TSCs and P10 TSCs. Scale bar = 1 mm. The p -value was calculated by a two-tailed, unpaired Student’s t-test (a–d) or one-way ANOVA followed by Tukey’s multiple comparisons tests (f). Data are shown as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001
Article Snippet:
Techniques: Staining, Control, Cell Culture, Expressing, Two Tailed Test